If anyone ever asks you to recite the kreb's cycle from memory heres basically how it goes.
Prep step
Pyruvate undergoes oxidative decarboxylation and is combined with Coenzyme A in order to form acetyl-coenzyme A. This step is catalyzed by an enzyme complex consisting of three parts. One has ThiaminePyroPhosphate as a coenzyme, the next has lipoamine arms attached to the lysine of the enzyme, the last has FAD+ to accept electrons and then donate them to NAD+ to make NADH. The Final products of this step are: CO2, acetylCoA, and NADH
Step one:
oxaloacetate combines with the acetyl group from acetylCoA and H2O to form citrate, catalyzed by citrate synthase-aldol condensation
Step two:
Citrate is hydrated to cisAconitate and then degydrated to form Isocitrate, catalyzed by Aconitase Fe-S cluster
Step three:
Isocitrate then loses two hydrogens to NADP+ to form the intermediate oxalosuccinate which then undergoes beta-hydroxy decarboxylation to form alpha-ketoglutarate, losing a CO2, catalyzed by isocitrate dehydrogenase.
to be continued
Prep step
Pyruvate undergoes oxidative decarboxylation and is combined with Coenzyme A in order to form acetyl-coenzyme A. This step is catalyzed by an enzyme complex consisting of three parts. One has ThiaminePyroPhosphate as a coenzyme, the next has lipoamine arms attached to the lysine of the enzyme, the last has FAD+ to accept electrons and then donate them to NAD+ to make NADH. The Final products of this step are: CO2, acetylCoA, and NADH
Step one:
oxaloacetate combines with the acetyl group from acetylCoA and H2O to form citrate, catalyzed by citrate synthase-aldol condensation
Step two:
Citrate is hydrated to cisAconitate and then degydrated to form Isocitrate, catalyzed by Aconitase Fe-S cluster
Step three:
Isocitrate then loses two hydrogens to NADP+ to form the intermediate oxalosuccinate which then undergoes beta-hydroxy decarboxylation to form alpha-ketoglutarate, losing a CO2, catalyzed by isocitrate dehydrogenase.
to be continued